Subproject B1 – In situ mechanisms of polysaccharide degradation of key bacteroidetal genera in spring algae blooms

Responsible: Dörte Becher, Ernst-Moritz-Arndt University, Greifswald & Bernhard Fuchs, Max-Planck-Institut für Marine Mikrobiologie, Bremen

In frame of the BMBF-funded project MIMAS (www.mimas-project.de) proteomics methods have been generally adapted to the challenges of highly complex samples. In the result we were able to get first insights into the dynamic response of the bacterioplankton community to a spring diatom bloom. However, due to the high complexity of the samples, only a general overview about the dynamics could be achieved (Teeling, Fuchs et al. 2012 Science). To understand the interplay within the microbial community, more detailed information is necessary. Consequently, the overall objective of this project is to gain a deeper understanding of the in situ mechanisms of polysaccharide degradation of key bacteroidetal genera in spring algae blooms. Therefore, the determination of recurrence of blooming bacterial species after spring diatom blooms as well as the determination of the metabolic potential of bacterial key-organisms within this bacterial succession and finally the determination of the dynamic adaptation on proteome level within dominant bacterial groups will be in focus of this project.

 

 

Dynamic composition of the bacterioplankton in response to the diatom bloom in the North Sea in 2009 based on metaproteome data (visualization by J. Bernhardt)